Session: Session 5

A bio- and MS-guided approach to convert Belgian endive co-products for antioxidant ingredient Innovation

Maxime DE SOUSA LOPES MOREIRA¹, Pauline BRUNIAUX^1,2, Cyril RAVESCHOT², Christophe FLAUHAUT¹, François COUTTE¹, Benoit CUDENNEC¹

¹UMRt BioEcoAgro N° 1158, Univ. Lille, INRAe, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d'Opale, ICV-Institut Charles Viollette, 59000 Lille, France. , Lille, France
²VF-Bioscience, Parc Eurasanté, Loos-lez-Lille, France., Loos-lez-Lille, France

Belgian endive (Cichorium intybus var. foliosum) is an Asteraceae mainly produced in the north of France with 124,400 tons per year. Its production generates a lot of co-products like roots after forcing or endive peelings (Anon s. d.). Belgian endive is rich in inulin, and in specialized metabolites like polyphenols and lactone sesquiterpenes. The latter are responsible for bitterness and display different biological activities such as anti-inflammatory or anti-tumoral. Belgian endive polyphenols are particularly hydroxycinnamic acids such as chicoric, chlorogenic, caffeic, or caftaric acid which are also presents in the co-products. Their antioxidant capacity can be improved by bioconversion.

Our goal was to develop, by bio-conversion, an antioxidant ingredient that exerts its activity after gastrointestinal digestion in the intestine. A supernatant of Belgian endive co-product was bio-converted using selected acid bacteria. The antioxidant activity of obtained supernatants was measured, before and after the INFOGEST protocol of in vitro simulated gastrointestinal digestion (Brodkorb et al. 2019; Fouré et al. 2018), using the DCFH2-DA cellular assay and analysed by HPLC-MS/MS (U3000 coupled to an Impact II, Bruker). Metaboscape was used to identify molecular mass of compounds statistically different in a condition to another.

Finally, the bio-conversion is an effective way to improve antioxidant activity of Belgian endive supernatant. Concomitantly, the MS analyses of these latter coupled to retreatment of MS-data by bioinformatics tool reveal the molecules differentially present between 2 conditions. Bio-converted supernatants could be used in different fields like in cosmetics, in animal feeding but above all in food industry.

Anon. s. d. .2023.(https://agreste.agriculture.gouv.fr/agreste-web/disaron/IraLeg23130/detail/).

Brodkorb André et al. 2019. doi: 10.1038/s41596-018-0119-1.

Fouré Marion et al. 2018. doi: 10.1021/acs.jafc.8b01055.