Session: Session 3
Obesity, adipose tissue ECM in mice
Introduction
Obesity is a complex disease altering the quality of life, requiring innovative therapies and approaches1. The extracellular matrix (ECM) components have a crucial influence in the adipose microenvironment. This network of proteins controls cell differentiation, migration, repair, survival, and development2, and its remodeling appears essential for healthy adipose tissue expansion. The composition of the adipose tissue matrisome is mainly known for its collagens, but identifying components other than those of the Core matrisome is necessary to define the signature of a healthy or unhealthy matrisome. Proteomic analyses of ECM proteins without prior enrichment are generally challenging due to the biochemical diversity of ECM components. This becomes complex for adipose tissues, which are very heterogeneous and rich in lipids.
Methods
We conducted a series of analyses on the perigonadal adipose tissue from lean and obese mice fed with a high fat diet for 2 months (n=6 in each group). After the tryptic digestion, peptides were separated by nanoLC before being analyzed by the Eclipse mass spectrometer. The evaluation of the strategy was based on the number of proteins identified/quantified and the number of ECM proteins, with reproducibility and repeatability.
Results
For all analyses, we identified 3634 proteins, with 2808 quantified and including 190 ECM proteins.These first analyses characterize the proteins of a healthy matrisome and a dysfunctional matrisome in the context of obesity-induced adipose tissue remodeling
Conclusion
We identified numerous differentially expressed ECM proteins core matrisome and associated-matrisome proteins, between the 2 groups of mice and these results confirmed that obesity lead to ECM remodeling.
1.Panuganti, K. K., Nguyen, M. & Kshirsagar, R. K. Obesity. StatPearls (2023)
2.Hynes, R. O. & Naba, A. Overview of the matrisome--an inventory of extracellular matrix constituents and functions. Cold Spring Harb. Perspect. Biol.(2012)
Methods
We conducted a series of analyses on the perigonadal adipose tissue from lean and obese mice fed with a high fat diet for 2 months (n=6 in each group). After the tryptic digestion, peptides were separated by nanoLC before being analyzed by the Eclipse mass spectrometer. The evaluation of the strategy was based on the number of proteins identified/quantified and the number of ECM proteins, with reproducibility and repeatability.
Results
For all analyses, we identified 3634 proteins, with 2808 quantified and including 190 ECM proteins.These first analyses characterize the proteins of a healthy matrisome and a dysfunctional matrisome in the context of obesity-induced adipose tissue remodeling
Conclusion
We identified numerous differentially expressed ECM proteins core matrisome and associated-matrisome proteins, between the 2 groups of mice and these results confirmed that obesity lead to ECM remodeling.
1.Panuganti, K. K., Nguyen, M. & Kshirsagar, R. K. Obesity. StatPearls (2023)
2.Hynes, R. O. & Naba, A. Overview of the matrisome--an inventory of extracellular matrix constituents and functions. Cold Spring Harb. Perspect. Biol.(2012)