Session: Parallel session 6 - Proteogenomics and metaproteomics

State of art and challenges in Metaproteomic

Metaproteomics, a scientific discipline studying proteins within microbial communities, sheds light on microorganism-environment interactions and underlying molecular mechanisms. Using mass spectrometry, researchers identify and quantify proteins in complex samples, analyzing data to understand microbial community composition and protein functions. Metaproteomics presents enduring challenges, encompassing the intricate preparation of complex matrix samples, the acquisition of thousands of peptides via mass spectrometry, and the intricate bioinformatic processing involving vast databases.

Metaproteomics finds diverse practical applications, notably in assessing the impact of pollutants on aquatic ecosystems in the environment, enhancing agricultural crop productivity, and promoting human health. Metaproteomics holds promise in disease research suc as in Inflammatory bowel disease, obesity, by offering insights into the communication mechanisms between the human host and the microbiome.

The evolution of mass spectrometry techniques, particularly in metaproteomics, has been remarkable. While traditional methods like Data-Dependent Acquisition (DDA) laid the foundation for protein identification, they are limited when dealing with complex samples like those in microbial communities. The efficient mass spectrometry acquisition methods, such as ion mobility and Data-Independent Acquisition (DIA), using modern mass spectrometers will be discussed. These advancements hold promise for uncovering novel biological insights and addressing complex challenges in health, ecology, and agriculture.